THE O-MANNOSYLATION AND PRODUCTION OF RECOMBINANT APA (45/47 KDA) PROTEIN FROM MYCOBACTERIUM TUBERCULOSIS IN STREPTOMYCES LIVIDANS IS AFFECTED BY CULTURE CONDITIONS IN SHAKE FLASKS

The O-mannosylation and production of recombinant APA (45/47 KDa) protein from Mycobacterium tuberculosis in Streptomyces lividans is affected by culture conditions in shake flasks

Abstract Background The Ala-Pro-rich O-glycoprotein known as the 45/47 kDa or APA antigen from Mycobacterium tuberculosis is an immunodominant adhesin restricted to mycobacterium genus and has been proposed as an alternative candidate to generate a new vaccine against tuberculosis or for diagnosis kits.In this work, the recombinant O-glycoprotein A

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